WEB-REPORT
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Group 1-11
Project Title Synthesis of biobutanol from watermelon peel
Synopsis Butanol has been found to have the potential to replace traditional gasoline. Watermelon peels have shown to have great potential as a novel feedstock for the production of butanol due to its high cellulose content. The aim of this study is to investigate the viability of using watermelon peels to produce butanol via ABE fermentation by Clostridium Acetobutylicum ATCC 824, as well as to investigate the effectiveness of different methods of producing butanol using watermelon peels. The watermelon peels were subjected to different types of pretreatment processes (sulfuric acid, sodium hydroxide and hot water pretreatment). The pretreated peels were then subjected to different forms of hydrolysis (acid and enzymatic hydrolysis) using 0.5M sulfuric acid or enzymes cellulase and cellobiase respectively. Each experiment was then tested for glucose yield after hydrolysis. The hydrolysate sample with the highest glucose yield, which was hot water pretreatment and enzymatic hydrolysis with an average glucose yield of 24.7g/L, underwent fermentation by C. Acetobutylicum for 168 hours to obtain an average of 1.47g/L of butanol. In this study, results show that enzymatic hydrolysis generally results in a higher glucose yield compared to acid hydrolysis, and hot water pretreatment combined with enzymatic hydrolysis gives rise to the highest glucose yield. The results also show that watermelon peels are a viable feedstock for the production of butanol via fermentation by C. Acetobutylicum. (224 words)
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Team Members
(Names & Classes)
Group Leader:
LIM KONG KIAT          3S1
 
Group Member/s:
LAW YU CHEN          3S2
YANG QIFENG          3S2