WEB-REPORT
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Group 1-09
Project Title Removal of biofilms using extract of plants and algae
Synopsis A biofilm is a group of bacterial cells that are surrounded in an extracellular matrix. Bacterial biofilms often adhere to surfaces and are difficult to eradicate using conventional antimicrobial agents. This study aims to investigate the ability of extracts of plants and algae to remove or inhibit formation of biofilms of the bacteria Pseudomonas putida and Pseudomonas fluorescens. Tests included the well diffusion assay, swimming and swarming motility assay, and the crystal violet assay for biofilm removal and inhibition of biofilm formation. The findings revealed that all extracts were not antibacterial towards P. putida and P. fluorescens in the well diffusion test; however, some extracts, especially cranberry, resulted in a reduction in the swimming and swarming motility of both bacteria tested, suggesting that these extracts reduced the quorum sensing of the bacteria, and hence, biofilm formation. Halimeda extract inhibited the formation of P. putida biofilm in the crystal violet assay. Thus these extracts are potential agents that can be applied large-scale to reduce the problem of widespread biofilm formation.
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Team Members
(Names & Classes)
Group Leader:
CHRISTOPHER ONG XIANBO 3S1
Group Member/s:
CHAN JIN KAI 3S1
NEOH JING HERNG 3S1